Journal: Materials Today Bio

Article Title: Injectable anti-inflammatory, antioxidant supramolecular nanofiber hydrogel for peripheral nerve injury repair and neuropathic pain relief

doi: 10.1016/j.mtbio.2026.102780

Figure Lengend Snippet: Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), Gap43 immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: The antibodies used and their dilution ratio include: NF-κB (CST, 1:1000), P- NF-κB (CST, 1:1000), Gap43 (CST, 1:1000), S100β (Abcam, 1:1000), GAPDH (Proteintech, 1:1000).

Techniques: Staining, Immunohistochemistry, Western Blot, Expressing, Software